Sperm Freezing 

Assisted reproductive technology has achieved a higher success rate with the application of different advanced techniques. In the field of embryology, with the introduction of cryopreservation, the success rates of IVF cycles dramatically improved.  Cryopreservation simply means preserving cells and tissues in live conditions where the cell metabolism comes to a standstill. Now it is routinely practiced in the form of freezing embryos, freezing sperms, or freezing oocytes.  

Cryoprecipitate 

Different permeable and nonpermeable substances are used for freezing. Ethylene glycol and Dimethyl sulfoxide combination are most commonly used in ART techniques. 

Cryopreservation of sperms: Indication 

  1. before ART cycle in case partner is absent on the day of ovum pick up. 
  2. male partners before undergoing any malignancy treatment such as chemotherapy or radiotherapy.
  3. in case of severe male factor infertility where the ejaculate contains a very small number of sperms.
  4. in case of obstructive azoospermia where we extract sperms through TESA or PESA  and then freeze it for use during ovum pick up.

Procedure 

It can be done either by non-controlled rate cryopreservation or controlled rate cryopreservation. Whatever be the technique the viral parameters should be checked and consent must be taken from the couple and the sample should be collected by masturbation in a sterile container. Before the use of the frozen sample, it should be thawed and used.

SPERM VD 

Cryopreservation of sperm cells is mandatory in cases of azoospermia/severe oligozoospermia.  The routine methods for sperm freezing are not suitable for freezing small numbers of spermatozoa.  Most of the sperm cells will not be found after thawing in cases of rare spermatozoa. SPERM VD  is a novel solution of cryopreservation of very low numbers of spermatozoa giving us maximum fertility outcome. It is an optimal device for freezing small numbers of spermatozoa by which we can avoid unnecessary THESE procedures.

Advantages-Optimal recovery of cells. 

  • No loss of cells after thawing.  
  • The motility and viability of the thawed cell are unaffected.  
  • Optimal cryopreservation after TESE. 
  • the procedure will avoid unnecessary surgical sperm recovery. 
  • Males with severe oligozoospermia will be able to preserve their fertility potential.  
  • Easy use for the IVF laboratory staff.