Assisted reproductive Technology
Sperm freezing
The assisted reproductive Technology has achieved its higher success with different technique optimize the field of embryology cryopreservation dramatically improved the success rates of IVF cycles. Cryopreservation simply means preserving cells and tissues in live conditions where the cell metabolism comes to a standstill. Now it is routinely practiced in the form of freezing embryo ,freezing sperms or freezing oocytes.
Cryoprecipitate
Tees are different permeable aur non permeable substance that are used for slow freezing or ethylene glycol and dimethyl sulfoxide combination are most commonly used in ART techniques.
Cryopreservation of sperms
Indication
- Prior to ART cycle in case partner is absent on the day of ovum pick up.
- Male partners before undergoing any malignancy treatment such as chemotherapy or radiotherapy.
- In case of severe male factor infertility where the ejaculate contains a very small number of sperms.
- In case of obstructive azoospermia where buy TESA or PESA we extract the sperm and then freeze it.
Procedure
It can be done by non controlled rate cryopreservation are controlled rate cryopreservation. Whatever
be the technique vir the viral parameter should be checked and consent must be taken from the couple
and the sample should be collected by masturbation in a sterile container.
Before use of the frozen sample it should be thawed and used.